Ni-NTA -Nanogold

Ni-NTA-Nanogold®

 

Ni-NTA-Nanogold® is now available in two sizes: the original 1.8 nm gold size, and our new 5 nm Ni-NTA-Nanogold®, designed for easy visualization in the electron microscope without silver or gold enhancement.

Ni-NTA-Nanogold® is designed for detection or localization of polyhistidine (his) -tagged fusion proteins using electron microscopy, light microscopy or blotting.

Using Ni-NTA-Nanogold®, his-tagged fusion proteins originating from any of a variety of expression vectors can be labeled under non-denaturing or denaturing conditions. The labeled his-tagged fusion proteins can be visualized by microscope or blots, when used with gold or silver enhancement reagents such as our Gold Enhance EM (Catalog number 2113), Gold Enhance LM (Catalog number 2112), or HQ Silver (Catalog Number 2012).

 Ni-NTA-Nanogold® is designed for detection and localization of polyhistidine-tagged proteins using electron microscopy, light microscopy and blotting.

Ni-NTA-Nanogold® comprises a 1.8 nm or 5 nm Nanogold® particle with multiple nickel-nitrilotriacetic acid functionalities incorporated into the ligands on the surface of gold particles. Nickel-nitrilotriacetic acid functionalities bind to histidines from the tagged proteins, and form stable complexes with extremely low dissociation constants.

Using Ni-NTA-Nanogold®, His-tagged fusion proteins originating from any of a variety of expression vectors can be labeled under both non-denaturing and denaturing conditions. By using silver or gold enhancement, The labeled his-tagged proteins can be visualized in the electron or light microscope and on blots.


Structure and STEM (39k)