Bioxytech GSH/GSSH-412 a colorimetric determination assay kit of detection of Reduced and Oxidized Glutathione 

click here to view the description and protocol of the assay.

Introduction:

Reduced glutathione (GSH), a tripeptide (g-glutamylcysteinylglycine) with a free thiol group, is a major antioxidant in human tissues that provides reducing equivalents for the glutathione peroxidase (GPx) catalyzed reduction of hydrogen peroxide and lipid hydroperoxides to water and the respective alcohol. During this process GSH becomes oxidized glutathione (GSSG). The GSSG is then recycled to GSH by reduction by the reduced form of beta-nicotinamide adenine dinucleotide phosphate (NADPH), catalyzed by glutathione reductase (GR). When mammalian cells are exposed to increased oxidative stress, the ratio of GSH/GSSG will decrease as a consequence of GSSG accumulation. The measure of the GSSG level, or determining the GSH/GSSG ratio, is a useful measure of oxidative stress and to monitor the effectiveness of antioxidant intervention strategies.

Assay Precision: