Ni-NTA -Nanogold

Ni-NTA-Nanogold^®

^{Ni-NTA-Nanogold® is
now available in two sizes: the original 1.8 nm gold size, and our new
5 nm Ni-NTA-Nanogold®, designed for easy visualization in
the electron microscope without silver or gold enhancement.
Ni-NTA-Nanogold® is
designed for detection or localization of polyhistidine (his) -tagged fusion
proteins using electron microscopy, light microscopy or blotting.
Using
Ni-NTA-Nanogold®, his-tagged fusion proteins originating from any of a
variety of expression vectors can be labeled under non-denaturing or
denaturing conditions. The labeled his-tagged fusion proteins can be
visualized by microscope or blots, when used with gold or silver enhancement
reagents such as our Gold Enhance
EM (Catalog number 2113), Gold Enhance
LM (Catalog number 2112), or HQ Silver (Catalog
Number 2012).
Ni-NTA-Nanogold® is
designed for detection and localization of polyhistidine-tagged proteins
using electron microscopy, light microscopy and blotting.
Ni-NTA-Nanogold® comprises
a 1.8 nm or 5 nm Nanogold® particle with multiple nickel-nitrilotriacetic
acid functionalities incorporated into the ligands on the surface of gold
particles. Nickel-nitrilotriacetic acid functionalities bind to histidines
from the tagged proteins, and form stable complexes with extremely low
dissociation constants.
Using
Ni-NTA-Nanogold®, His-tagged fusion proteins originating from any of a
variety of expression vectors can be labeled under both non-denaturing and
denaturing conditions. By using silver or gold enhancement, The labeled
his-tagged proteins can be visualized in the electron or light microscope and
on blots.}